Composite

Part:BBa_K4350829

Designed by: Ryan Riopel   Group: iGEM22_UBC-Okanagan   (2022-10-09)


nnLuz V49A Transcriptional Unit

Transcriptional unit for the expression of N. nambi fungal luciferase with a V49A mutation. Assembled as a level 1 MoClo part compatible for assembly into a level 2 multi-gene construct. Composed of the PlacIQ synthetic constitutive promoter based on the PLacI promoter from E. coli, the nnLuz V49A coding sequence, and Tcpc terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None